KMID : 0379120150430030200
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Korean Journal of Mycology 2015 Volume.43 No. 3 p.200 ~ p.205
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Plant Growth Promotion and Gibberellin A3 Production by Aspergillus flavus Y2H001
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You Young-Hyun
Park Jong-Myong Kang Sang-Mo Park Jong-Han Lee In-Jung Kim Jong-Kuk
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Abstract
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Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new GA3-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.
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KEYWORD
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Aspergillus flavus, Endophytic fungi, Gibberellic acid, Plant growth promotion
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